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OPTIMIZATION OF PAPAIN EXTRACTION BY AQUEOUS TWO-PHASE SYSTEM (ATPS) USING RESPONSE SURFACE METHODOLOGY (RSM)

Chaman Mehta.C.M. P. Rajesh V.Sridevi , Divya M. L and Satya Ch.V

This work attempts to study and optimize aqueous two-phase system (ATPS) made of PEG, sodium carbonate and PEG, sodium sulphate. Three factors ;PEG concentration, salt concentration and pH affecting the papain partitioning were studied. The statistical analysis showed that for both systems the phase forming salt concentration significantly affect the “K” value for papain partitioning. However, the performance of PEG/Na2CO3 system was generally better than PEG/Na2SO4 system. Hence, detail study was carried out on the PEG/Sodium carbonate system using CCRD (Central Composite Rotatable Design) in Response Surface Methodology (RSM). The optimal condition gave a “K” value of partitioning of 1.51 with a yield of 76%.