S M Gopinath
The main aim of this study is to investigate about citric acid production by ETGP12 and ETGP18 strains of Aspergillus niger with the inclusion of certain nutrients along with the substrates. The substrates (rice chaff and sesamum oil cake) were fermented in the presence of different nutrients like ammonium carbonate and potassium dihydrogen phosphate at concentrations ranging from 0.1 to 0.5 mg/100g of substrates for a period of 72 and 96 hours and the same were tested even in mixed combinations of nutrients. In both the cases it was observed that these nutrients enhanced the rate of citric acid production initially but at higher concentrations the yield of citric acid is reduced.