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A High-Sensitivity Analysis Method of Furanocoumarins in Radix Angelicae Dahuricae by Reversed-Phase High-Performance Liquid Chromatography Coupled with Integrated Pulsed Amperometric Detection

Seon-Pyo Hong, Young-Ju Jung, Min-Ji Lee

Radix Angelicae dahuricae is a traditional Chinese medicine. We developed a high-sensitivity method for detection of furanocoumarins in Radix Angelicae dahuricae and Gumiganghwal-tang (GMGHT). The six furanocoumarins of Radix Angelicae dahuricae were sonication-extracted from 50% ethanol for 60 min. Six furanocoumarins were separated through a gradient elution system. The limits of detection of the components were 0.002–0.3 ng (0.2–30 ng/mL). The coefficients of determination were 0.9995–1.0000, all inter-day and intra-day precision values were<4.9%, and the mean recoveries and relative standard deviations were 96.4%–104.5% and 0.5%–4.8% for Radix Angelicae dahuricae extract, respectively. Our method does not require any pretreatment steps and exhibits good reproducibility, selectivity, and sensitivity. Therefore, our method will contribute to a Radix Angelicae dahuricae quality control measure.