Abstrait

First Derivative Spectroscopic Method for Simultaneous Estimation of Edaravone and Citicoline Sodium in Synthetic Mixture.

Bhumi K Patel, Hasumati A Raj, and Vineet C Jain

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Edaravone and Citicoline Sodium in synthetic mixture using first order derivative zero-crossing method. Edaravone showed zero crossing point at 245.60 nm while Citicoline Sodium showed zero crossing point at 271.20 nm. The dA/dλ was measured at 271.20 nm for Edaravone and 271.20nm for Citicoline Sodium and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.999) in the range of 1-6μg/ml for Edaravone at 271.20 nm. The linear correlation was obtained (r2>0.999) in the range of 25-150 μg/ml for Citicoline Sodium at 271.20 nm. The limit of determination was 0.032μg/ml and 0.831μg/ml for Edaravone and Citicoline Sodium, respectively. The limit of quantification was 0.098 μg/ml and 2.520 μg/ml for Edaravone and citicoline Sodium, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Edaravone and Citicoline Sodium in binary mixture.